Pubblicazioni

Functional characterization of the ribonucleoprotein, PTB-binding 1/Raver1 promoter region  (2007)

Autori:
Romanelli MG; Lorenzi P; Avesani F.; Morandi C.
Titolo:
Functional characterization of the ribonucleoprotein, PTB-binding 1/Raver1 promoter region
Anno:
2007
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
Gene
ISSN Rivista:
0378-1119
N° Volume:
405
Editore:
Elsevier
Intervallo pagine:
79-87
Parole chiave:
hnRNP; PTB; Promoter analysis; gene expression; splicing; ribonucleoproteins
Breve descrizione dei contenuti:
Raver1 is a ribonucleoprotein, evolutionarily conserved in mammals, which acts as a polypyrimidine tract-binding protein (PTB/hnRNPI) co-repressor in regulating alternative splicing events. The mouse homologue has been identified as a dual compartment protein that interacts with PTB within perinucleolar structures, and localizes at microfilament plasma membrane attachment sites in fibroblasts, epithelial and muscle cells. Human Raver1 gene is localized on chromosome 19p13.2 and encodes for an inferred 756 amino acid protein sharing 87% similarity with the mouse orthologue. The human Raver1 gene expression has not been previously investigated. Here we report the mRNA expression profile of human Raver1 gene and the molecular characterization of its promoter region. From the in silico analysis of 1542 bp of the Raver1 5'-flanking region (GC content=61%), no canonical TATA or CAAT boxes can be highlighted, whereas several consensus Sp1 putative binding sequences can be predicted within 1 kb from the transcription start site (TSS) that we determined by 5'-RACE. Functional analyses established a minimal region involved in the regulation of the human Raver1 promoter activity. Mutational analyses and transfection studies indicated that a GGGAGCTCCC sequence at -531 represents a putative cis signal acting as a negative regulator element of the promoter function. Altogether, our results indicate that human Raver1 gene promoter region shares common features of ubiquitously expressed gene with the interacting splicing regulator PTB.
Pagina Web:
http://www.ncbi.nlm.nih.gov/pubmed/17931803
Id prodotto:
39006
Handle IRIS:
11562/311896
depositato il:
21 febbraio 2012
ultima modifica:
22 giugno 2018
Citazione bibliografica:
Romanelli MG; Lorenzi P; Avesani F.; Morandi C., Functional characterization of the ribonucleoprotein, PTB-binding 1/Raver1 promoter region «Gene» , vol. 4052007pp. 79-87

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