Publications

Authors:

Monaco, Salvatore; Jacob, J.; Jenich, H.; Patton, A.; Autilio Gambetti, L.; Gambetti, P.

Title:

Axonal transport of neurofilament is accelerated in peripheral nerve during 2,5-hexanedione intoxication.

Year:

1989

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Brain Research

ISSN of journal:

0006-8993

N° Volume:

491

Number or Folder:

2

Page numbers:

328-334

Keyword:

Axonal transport; 2; 5-Hexanedione; Neurofilament; Giant axonal neuropathy; Acceleration of transport

Note:

The neurotoxic compound 2,5-hexanedione (2,5-HD) causes an axonopathy characterized by the presence of neurofilament (NF)-containing enlargements in the preterminal segments of central and peripheral axons. The 2,5-HD axonopathy is a good model for human acquired and inherited giant axonal neuropathies. Recently, we reported that following 2,5-HD administration, axonal transport of NF is markedly and selectively accelerated in the primary visual system. We have now studied slow axonal transport in the sciatic system of rats intoxicated with 0.5% 2,5-HD in drinking water. Following radiolabeling, transported proteins were examined after polyacrylamide gel electrophoresis and fluorography. The bulk of radiolabeled NF subunits was located 30–50 mm from the spinal cord in 2,5-HD treated animals and 10–25 mm in controls. The rate of transport of the three NF subunits was 0.7 mm/day in controls and 1.2 mm/day in 2,5-HD treated animals. The rate of transport of tubulin was not significantly changed. Electrophysiological studies of soleus nerve and muscle showed no evidence of denervation after 6 weeks of intoxication. It is concluded that, following 2,5-HD administration, transport of NF is preferentially accelerated in both central and peripheral axons. A pathogenetic mechanism based on the acceleration of NF transport is proposed, which may explain the formation and the distal or proximal location of NF-containing axonal enlargements in giant axonopathies.

Web page:

http://dx.doi.org/10.1016/0006-8993(89)90067-X

Product ID:

51626

Handle IRIS:

11562/231595

Last Modified:

November 11, 2022

Bibliographic citation:

Monaco, Salvatore; Jacob, J.; Jenich, H.; Patton, A.; Autilio Gambetti, L.; Gambetti, P., Axonal transport of neurofilament is accelerated in peripheral nerve during 2,5-hexanedione intoxication. «Brain Research» , vol. 491 , n. 2 , 1989 , pp. 328-334

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