Flow Cytometry (2020/2021)

Course code
cod wi: DT000123
Name of lecturer
Maria Scupoli
Coordinator
Maria Scupoli
Number of ECTS credits allocated
2.25
Academic sector
BIO/13 - EXPERIMENTAL BIOLOGY
Language of instruction
Italian
Location
VERONA
Period
A.A. 20/21 dottorato dal Oct 1, 2020 al Sep 30, 2021.

Lesson timetable

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Learning outcomes

The knowledge of basic principles and main applications of flow cytometry.

Syllabus

Fundamental principles of flow cytometry: the history of flow cytometry; instrument components; light-matter interaction; fluorochromes; optics; electronics and data analysis concepts.
Multidimensional flow cytometry: data analysis and presentation; the power of multiparameter flow cytometry.
Functional flow cytometry: cell sorting; cell cycle; intracellular calcium; apoptosis; cell signaling; translational applications.

Reference books
Author Title Publisher Year ISBN Note
Claudio Ortolani Citometria a flusso edi-ermes 2019 ISBN 978-88-7051-693-7

Assessment methods and criteria

Active participation to the meetings is mandatory. There is no further evaluation.