Publications

Authors:

Mariotto, Sara; Gajofatto, Alberto; Batzu, Lucia; Delogu, Rachele; Sechi, Gianpietro; Leoni, Stefania; Pirastru, Maria Immacolata; Bonetti, Bruno; Zanoni, Mattia; Alberti, Daniela; Schanda, Kathrin; Monaco, Salvatore; Reindl, Markus; Ferrari, Sergio

Title:

Relevance of antibodies to myelin oligodendrocyte glycoprotein in CSF of seronegative cases

Year:

2019

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

NEUROLOGY

ISSN of journal:

0028-3878

N° Volume:

93

Number or Folder:

20

Page numbers:

e1867-e1872

Keyword:

myelin oligodendrocyte glycoprotein; antibody; CSF; seronegative patients; diagnostic sensitivi

Short description of contents:

OBJECTIVE: To determine the diagnostic relevance of myelin oligodendrocyte glycoprotein antibodies (MOG-Abs) in CSF of seronegative cases by retrospectively analyzing consecutive time-matched CSF of 80 MOG-Ab-seronegative patients with demyelinating disease. METHODS: The cohort included 44 patients with NMOSD and related disorders and 36 patients with multiple sclerosis (MS). Two independent neurologists blinded to diagnosis analyzed MOG-Abs by live cell-based immunofluorescence assay with goat anti-human immunoglobulin (Ig) G (whole molecule) antibody. Sera were tested at dilutions of 1:20 and 1:40, and a cutoff of 1:160 was considered for serum positivity. CSF specimens were tested undiluted and at 1:2 dilution with further titrations in case of positivity. Anti-IgG-Fc and anti-IgM-µ secondary antibodies were used to confirm the exclusive presence of MOG-IgG in positive cases. CSF of 13 MOG-Abs seropositive cases and 36 patients with neurodegenerative conditions was analyzed as controls. RESULTS: Three seronegative cases had CSF MOG-Abs (4% of the whole cohort or 7% of cases excluding patients with MS, in which MOG-Abs seem to lack diagnostic relevance). In particular, 2 patients with neuromyelitis optica spectrum disorder (NMOSD) and 1 with acute disseminated encephalomyelitis had MOG-Abs in CSF. Analysis with anti-IgG-Fc and anti-IgM confirmed the exclusive presence of MOG-IgG in the CSF of these patients. Among the control group, MOG-Abs were detectable in the CSF of 8 of 13 MOG-Ab-seropositive cases and in none of the patients with neurodegenerative disorders. CONCLUSION: Although serum is the optimal specimen for MOG-Ab testing, analyzing CSF could improve diagnostic sensitivity in seronegative patients. This observation has relevant diagnostic impact and might provide novel insight into the biological mechanisms of MOG-Ab synthesis.

Web page:

https://doi.org/10.1212/WNL.0000000000008479

Product ID:

110912

Handle IRIS:

11562/1001975

Last Modified:

November 29, 2022

Bibliographic citation:

Mariotto, Sara; Gajofatto, Alberto; Batzu, Lucia; Delogu, Rachele; Sechi, Gianpietro; Leoni, Stefania; Pirastru, Maria Immacolata; Bonetti, Bruno; Zanoni, Mattia; Alberti, Daniela; Schanda, Kathrin; Monaco, Salvatore; Reindl, Markus; Ferrari, Sergio, Relevance of antibodies to myelin oligodendrocyte glycoprotein in CSF of seronegative cases «NEUROLOGY» , vol. 93 , n. 20 , 2019 , pp. e1867-e1872

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