Pubblicazioni

Autori:

Brennan, Gary P; Bauer, Sebastian; Engel, Tobias; Jimenez-Mateos, Eva M; Del Gallo, Federico; Hill, Thomas D M; Costard, Lara S; Neubert, Valentin; Salvetti, Beatrice; Sanz-Rodriguez, Amaya; Heiland, Mona; Mamad, Omar; Brindley, Elizabeth; Norwood, Braxton; Batool, Aasia; Raoof, Rana; El-Naggar, Hany; Connolly, Niamh M C; Reschke, Cristina; Delanty, Norman; Prehn, Jochen H M; Fabene, Paolo; Mooney, Catherine; Rosenow, Felix; Henshall, David C

Titolo:

Genome-wide microRNA profiling of plasma from three different animal models identifies biomarkers of temporal lobe epilepsy

Anno:

2020

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

NEUROBIOLOGY OF DISEASE

ISSN Rivista:

0969-9961

N° Volume:

144

Numero o Fascicolo:

105048

Editore:

Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445

Intervallo pagine:

1-13

Parole chiave:

biomarkers; epilepsy; microRNA

Breve descrizione dei contenuti:

Epilepsy diagnosis is complex, requires a team of specialists and relies on in-depth patient and family history, MRI-imaging and EEG monitoring. There is therefore an unmet clinical need for a non-invasive, molecular-based, biomarker to either predict the development of epilepsy or diagnose a patient with epilepsy who may not have had a witnessed seizure. Recent studies have demonstrated a role for microRNAs in the pathogenesis of epilepsy. MicroRNAs are short non-coding RNA molecules which negatively regulate gene expression, exerting profound influence on target pathways and cellular processes. The presence of microRNAs in biofluids, ease of detection, resistance to degradation and functional role in epilepsy render them excellent candidate biomarkers. Here we performed the first multi-model, genome-wide profiling of plasma microRNAs during epileptogenesis and in chronic temporal lobe epilepsy animals. From video-EEG monitored rats and mice we serially sampled blood samples and identified a set of dysregulated microRNAs comprising increased miR-93-5p, miR-142-5p, miR-182-5p, miR-199a-3p and decreased miR-574-3p during one or both phases. Validation studies found miR-93-5p, miR-199a-3p and miR-574-3p were also dysregulated in plasma from patients with intractable temporal lobe epilepsy. Treatment of mice with common anti-epileptic drugs did not alter the expression levels of any of the five miRNAs identified, however administration of an anti-epileptogenic microRNA treatment prevented dysregulation of several of these miRNAs. The miRNAs were detected within the Argonuate2-RISC complex from both neurons and microglia indicating these miRNA biomarker candidates can likely be traced back to specific brain cell types. The current studies identify additional circulating microRNA biomarkers of experimental and human epilepsy which may support diagnosis of temporal lobe epilepsy via a quick, cost-effective rapid molecular-based test.

Pagina Web:

https://doi.org/10.1016/j.nbd.2020.105048

Id prodotto:

115796

Handle IRIS:

11562/1023166

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Brennan, Gary P; Bauer, Sebastian; Engel, Tobias; Jimenez-Mateos, Eva M; Del Gallo, Federico; Hill, Thomas D M; Costard, Lara S; Neubert, Valentin; Salvetti, Beatrice; Sanz-Rodriguez, Amaya; Heiland, Mona; Mamad, Omar; Brindley, Elizabeth; Norwood, Braxton; Batool, Aasia; Raoof, Rana; El-Naggar, Hany; Connolly, Niamh M C; Reschke, Cristina; Delanty, Norman; Prehn, Jochen H M; Fabene, Paolo; Mooney, Catherine; Rosenow, Felix; Henshall, David C, Genome-wide microRNA profiling of plasma from three different animal models identifies biomarkers of temporal lobe epilepsy «NEUROBIOLOGY OF DISEASE» , vol. 144 , n. 105048 , 2020 , pp. 1-13

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