Publications

Authors:

Costanzo, Manuela; Esposito, Elisabetta; Sguizzato, Maddalena; Lacavalla, Maria Assunta; Drechsler, Markus; Valacchi, Giuseppe; Zancanaro, Carlo; Malatesta, Manuela

Title:

Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery

Year:

2021

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

Elettronico

Referee:

Sì

Name of journal:

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN of journal:

1422-0067

N° Volume:

22

Number or Folder:

5341

Page numbers:

1-20

Keyword:

cell culture; cholecalciferol; cryogenic transmission electron microscopy; in vitro test; light microscopy; lipid nanocarriers; transmission electron microscopy

Short description of contents:

In this pilot study, ethosomes and transethosomes were investigated as potential delivery systems for cholecalciferol (vitamin D3), whose deficiency has been correlated to many disorders such as dermatological diseases, systemic infections, cancer and sarcopenia. A formulative study on the influence of pharmaceutically acceptable ionic and non-ionic surfactants allowed the preparation of different transethosomes. In vitro cytotoxicity was evaluated in different cell types representative of epithelial, connective and muscle tissue. Then, the selected nanocarriers were further investigated at light and transmission electron microscopy to evaluate their uptake and intracellular fate. Both ethosomes and transethosomes proven to have physicochemical properties optimal for transdermal penetration and efficient vitamin D3 loading; moreover, nanocarriers were easily internalized by all cell types, although they followed distinct intracellular fates: ethosomes persisted for long times inside the cytoplasm, without inducing subcellular alteration, while transethosomes underwent rapid degradation giving rise to an intracellular accumulation of lipids. These basic results provide a solid scientific background to in vivo investigations aimed at exploring the efficacy of vitamin D3 transdermal administration in different experimental and pathological conditions.

Web page:

https://doi.org/10.3390/ijms22105341

Product ID:

121297

Handle IRIS:

11562/1044503

Last Modified:

November 15, 2022

Bibliographic citation:

Costanzo, Manuela; Esposito, Elisabetta; Sguizzato, Maddalena; Lacavalla, Maria Assunta; Drechsler, Markus; Valacchi, Giuseppe; Zancanaro, Carlo; Malatesta, Manuela, Formulative Study and Intracellular Fate Evaluation of Ethosomes and Transethosomes for Vitamin D3 Delivery «INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES» , vol. 22 , n. 5341 , 2021 , pp. 1-20

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