Publications

Authors:

Ammendola, Serena; Caldonazzi, Nicolò; Simbolo, Michele; Piredda, Maria Liliana; Brunelli, Matteo; Poliani, Pietro Luigi; Pinna, Giampietro; Sala, Francesco; Ghimenton, Claudio; Scarpa, Aldo; Barresi, Valeria

Title:

H3K27me3 immunostaining is diagnostic and prognostic in diffuse gliomas with oligodendroglial or mixed oligoastrocytic morphology

Year:

2021

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

VIRCHOWS ARCHIV

ISSN of journal:

0945-6317

N° Volume:

479

Number or Folder:

5

:

Springer Verlag Germany:Tiergartenstrasse 17, D 69121 Heidelberg Germany:011 49 6221 3450, EMAIL: g.braun@springer.de, INTERNET: http://www.springer.de, Fax: 011 49 6221 345229

Page numbers:

987-996

Keyword:

1p/19q codeletion; Astrocytoma; H3K27me3; Oligodendroglioma; Recurrence

Short description of contents:

Oligodendroglioma is defined by IDH mutation and 1p/19q codeletion. The latter is mutually exclusive to ATRX immunohistochemical loss and has been recently associated with the loss of H3K27me3 immunostaining. We aimed to assess the diagnostic and prognostic value of H3K27me3 immuno-expression in diffuse gliomas with oligodendroglial or mixed oligoastrocytic morphology. H3K27me3 immunostaining was performed in 69 diffuse gliomas with oligodendroglial (n = 62) or oligoastrocytic (n = 7) morphology. The integration with routinely assessed IDH mutations, ATRX immunostaining, and 1p/19q codeletion classified these cases as 60 oligodendroglial and 9 astrocytic. H3K27me3 was lost in 58/60 oligodendrogliomas with retained (n = 47) or non-conclusive (n = 11) ATRX staining, 3/6 IDH-mutant astrocytomas with ATRX loss, and 3/3 IDH-wt astrocytomas. H3K27me3 was retained in 2/60 oligodendrogliomas with retained ATRX, and in 3/6 IDH-mutant astrocytomas, two of which had lost and one retained ATRX. The combination of H3K27me3 and ATRX immunostainings with IDH mutational status correctly classified 55/69 (80%) cases. In IDH-mutant gliomas, ATRX loss indicates astrocytic phenotype, while ATRX retention and H3K27me3 loss identify oligodendroglial phenotype. Only 14 (20%) IDH-mutant cases with retained ATRX and H3K27me3 or inconclusive ATRX immunostaining would have requested 1p/19q codeletion testing to be classified. Furthermore, H3K27me3 retention was associated with significantly shorter relapse-free survival (P < 0.0001), independently from IDH mutation or 1p/19q codeletion (P < 0.005). Our data suggest that adding H3K27me3 immunostaining to the diagnostic workflow of diffuse gliomas with oligodendroglial or mixed morphology is useful for drastically reducing the number of cases requiring 1p/19q codeletion testing and providing relevant prognostic information.

Note:

Aldo Scarpa and Valeria Barresi contributed equally to this work. Aldo Scarpa and Valeria Barresi shared last authorship.

Web page:

https://doi.org/10.1007/s00428-021-03134-1

Product ID:

121583

Handle IRIS:

11562/1045326

Last Modified:

November 14, 2022

Bibliographic citation:

Ammendola, Serena; Caldonazzi, Nicolò; Simbolo, Michele; Piredda, Maria Liliana; Brunelli, Matteo; Poliani, Pietro Luigi; Pinna, Giampietro; Sala, Francesco; Ghimenton, Claudio; Scarpa, Aldo; Barresi, Valeria, H3K27me3 immunostaining is diagnostic and prognostic in diffuse gliomas with oligodendroglial or mixed oligoastrocytic morphology «VIRCHOWS ARCHIV» , vol. 479 , n. 5 , 2021 , pp. 987-996

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