Pubblicazioni

Autori:

Pizzolo, Giovanni; Rigo, Antonella; Zanotti, Roberta; Vinante, Fabrizio; Vincenzi, C; Cassatella, Marco Antonio; Carra, Giuseppe; Castaman, G; Chilosi, Marco; Semenzato, G.

Titolo:

Alpha (p55) and beta (p75) chains of the interleukin-2 receptor are expressed by AML blasts

Anno:

1993

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Leukemia

ISSN Rivista:

0887-6924

N° Volume:

7

Intervallo pagine:

418-425

Parole chiave:

Acute leukemia; IL-2R; Leukemic growth

Breve descrizione dei contenuti:

In the present study, we have investigated the leukemic cells obtained from 16 patients with acute myeloid leukemia (AML) at diagnosis for the membrane expression of p55 (alpha) and p75 (beta) interleukin-2 receptor (IL-2R) chains using specific monoclonal antibodies (mAbs), as well as for the presence of their transcripts using Northern blot analysis. In addition, immunoprecipitation of the p75 membrane molecule with TU27 and Mik-beta 1 mAbs was carried out in selected cases. The p75 IL-2R beta transcripts were detected in all cases, whereas the membrane p75 molecule was demonstrable by flow cytometry in three cases. However, data from the immunoprecipitation analysis suggest that the lack of the p75 IL-2R detection by flow cytometry might be caused by the low density of molecules per cell rather than the fact that the specific mRNA is not translated into the p75 surface molecule. In addition, a consistent membrane positivity with an anti-p55/CD25 mAb, present on fresh uncultured blasts in 37.5% of the cases, became detectable after short-term culture in 75% of cases. In each individual case, a strict correlation was found between membrane CD25 reactivity and the expression of p55 mRNA. Taken together, our data suggest that the expression of both alpha (p55) and beta (p75) IL-2R molecules is a common feature of leukemic cells in AML, and provide new arguments for reassessing the possible role of IL-2 in leukemic growth.

Id prodotto:

2135

Handle IRIS:

11562/2135

depositato il:

27 dicembre 2011

ultima modifica:

13 novembre 2022

Citazione bibliografica:

Pizzolo, Giovanni; Rigo, Antonella; Zanotti, Roberta; Vinante, Fabrizio; Vincenzi, C; Cassatella, Marco Antonio; Carra, Giuseppe; Castaman, G; Chilosi, Marco; Semenzato, G., Alpha (p55) and beta (p75) chains of the interleukin-2 receptor are expressed by AML blasts «Leukemia» , vol. 7 , 1993 , pp. 418-425

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo