Publications

Authors:

Michelson, S; Dal Monte, P; Zipeto, D; Bodaghi, B; Laurent, L; Oberlin, E; Arenzana-Seisdedos, F; Virelizier, Jl; Landini, Mp

Title:

Modulation of RANTES production by human cytomegalovirus (CMV) infection of fibroblasts.

Year:

1997

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Journal of Virology

ISSN of journal:

0022-538X

N° Volume:

71

Number or Folder:

9

Page numbers:

6495-6500

Keyword:

Rantes cytomegalovirus fibroblasts

Short description of contents:

Chemokines play a major role in inflammatory responses and affect hematopoiesis both negatively and positively. We show that fresh isolates and laboratory strains (Towne and Ad-169) of human cytomegalovirus (HCMV) induce production of the CC chemokine RANTES in fibroblasts. Induction of extracellular RANTES production occurred as early as 8 h after infection, peaked around 24 h after infection, and was almost undetectable by 48 and 72 h. Upregulation occurred in the absence of viral DNA synthesis, suggesting that it was due to immediate-early-early HCMV gene expression. CMV infection stimulated RANTES transcription, since reverse transcription-PCR detected a sharp increase in RANTES RNA which persisted even when extracellular RANTES was no longer detected. Induction of RANTES in fibroblasts was not due to prior induction of tumor necrosis factor alpha or interleukin 1 beta. Down-regulation required an active viral genome. Decrease of RANTES in culture supernatants may be associated with the appearance of the HCMV CC chemokine receptor US28, since we show that this gene is transcribed as early as 8 h after infection. Modulation of CC chemokine production early during CMV infection might have a regulatory effect on viral replication, as well as affect immune surveillance.

Web page:

http://jvi.asm.org/content/71/9/6495.long
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC191924/?tool=pubmed

Product ID:

35256

Handle IRIS:

11562/236846

Deposited On:

June 22, 2012

Last Modified:

November 15, 2022

Bibliographic citation:

Michelson, S; Dal Monte, P; Zipeto, D; Bodaghi, B; Laurent, L; Oberlin, E; Arenzana-Seisdedos, F; Virelizier, Jl; Landini, Mp, Modulation of RANTES production by human cytomegalovirus (CMV) infection of fibroblasts. «Journal of Virology» , vol. 71 , n. 9 , 1997 , pp. 6495-6500

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