Pubblicazioni

Autori:

Mariotti, Raffaella; Tongiorgi, E.; Bressan, C.; Kristensson, K.; BENTIVOGLIO FALES, Marina

Titolo:

Priming by muscle inflammation alters the response and vulnerability to axotomy-induced damage of the rat facial motor nucleus

Anno:

2002

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Experimental Neurology

ISSN Rivista:

0014-4886

N° Volume:

176

Numero o Fascicolo:

1

Intervallo pagine:

133-142

Parole chiave:

Astrocytes; Bcl-2; Microglia; Motoneurons; Nerve transection; Nitric oxide synthase; T cell mitogens;

Breve descrizione dei contenuti:

To ascertain whether signaling due to peripheral inflammation affects motoneuron vulnerability, we examined in adult rats the reaction to axonal injury of facial motoneurons primed by muscle inflammation. In this double-hit paradigm, preconditioning was achieved by injections into the facial muscles of the T cell mitogen phytohemagglutinin, which was found in a previous study (11) to elicit a retrograde response in motoneurons. Facial nerve transection was used as test lesion. Intramuscular injections of saline prior to axotomy were used as control for lectin pretreatment. In rats pretreated with phytohemagglutinin injection, upregulation of the expression of the antiapoptotic bcl-2 gene, examined with in situ hybridization, was significantly higher in facial motoneurons at 2 days postaxotomy compared with saline-injected control cases. After repeated phytohemagglutinin injections followed by nerve transection, induction in facial motoneurons of nitric oxide synthase, revealed by histochemistry and immunohistochemistry, as well as activation of the surrounding microglia, was enhanced at 14 days postaxotomy with respect to the saline-treated control cases. At the same time point, no significant intergroup difference was detected in the intensity of astrocytic activation. At 1 month postaxotomy, stereological cell counts revealed that motoneuron loss was significantly greater in the cases pretreated with phytohemagglutinin than in the saline-treated cases. The data point out that the response of the facial motor nucleus to axonal damage is altered by previous exposure to peripheral inflammation and that such preconditioning stimulus enhances motoneuron vulnerability to nerve injury. © 2002 Elsevier Science (USA).

Id prodotto:

22612

Handle IRIS:

11562/304663

depositato il:

1 agosto 2007

ultima modifica:

15 novembre 2022

Citazione bibliografica:

Mariotti, Raffaella; Tongiorgi, E.; Bressan, C.; Kristensson, K.; BENTIVOGLIO FALES, Marina, Priming by muscle inflammation alters the response and vulnerability to axotomy-induced damage of the rat facial motor nucleus «Experimental Neurology» , vol. 176 , n. 1 , 2002 , pp. 133-142

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