Pubblicazioni

SK3 trafficking in hippocampal cells: the role of different molecular domains.  (2006)

Autori:
Decimo, Ilaria; Roncarati, R; Grasso, Silvia; Clemens, M; Chiamulera, Cristiano; Fumagalli, Guido Francesco
Titolo:
SK3 trafficking in hippocampal cells: the role of different molecular domains.
Anno:
2006
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
Nome rivista:
BIOSCIENCE REPORTS
ISSN Rivista:
0144-8463
N° Volume:
26
Editore:
Springer/Plenum Publishers, New York
Intervallo pagine:
399-412
Parole chiave:
SK3; IPPOCAMPO; IMMUNOFLUORESCENZA
Breve descrizione dei contenuti:
The regulative steps that control trafficking of ion channels are fundamental determinants of their qualitative and quantitative expression on the cell membrane. In this work the trafficking of the small conductance calcium-activated potassium channel, SK3 was studied in neurons in order to identify relevant molecular domains involved in this process. Hippocampal cell cultures were transfected with fusion proteins of green fluorescent protein (GFP) and different SK3 subunit truncations. The differential distribution of the mutants was analyzed by confocal microscopy and compared to the localization of the control fusion protein with full length SK3. The transport of chimeric proteins was quantified from fluorescence images by developing a morphometric analytical method. We found that the full length SK3 was distributed in cell body, axon and dendrites, whereas the deleted forms GFPDelta578-736 (deletion of the entire C-terminal domain), GFPDeltaCaMBD (deletion of the calmodulin-binding site) and GFPDeltaN (deletion of the N-terminal domain) were not transported into cell processes but accumulated in the cell body. The GFPDelta640-736 (deletion of the distal C-terminal domain) showed a distribution similar to control. The quantification and statistical analysis confirmed the differences in distribution across the three groups. In conclusion, the current work provides evidence for a fundamental role of the N-terminal domain and the calmodulin binding domain in SK3 trafficking in neurons.
Id prodotto:
36862
Handle IRIS:
11562/305875
depositato il:
6 marzo 2012
ultima modifica:
2 dicembre 2022
Citazione bibliografica:
Decimo, Ilaria; Roncarati, R; Grasso, Silvia; Clemens, M; Chiamulera, Cristiano; Fumagalli, Guido Francesco, SK3 trafficking in hippocampal cells: the role of different molecular domains. «BIOSCIENCE REPORTS» , vol. 262006pp. 399-412

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo IRIS

Progetti Collegati
Titolo Dipartimento Responsabili
Ruolo dell'attività e di fattori trofici nel controllo dell'espressione e della localizzazione subcellulare di canali al potassio a bassa conduttanza attivati dal calcio (SK3) nel muscolo scheletrico e in cellule nervose Dipartimento Ingegneria per la medicina di innovazione Guido Francesco Fumagalli
<<indietro

Attività

Strutture

Condividi