Pubblicazioni

Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro.  (2008)

Autori:
Elena, Bandi; Marko, Jevsek; Tomaz, Mars; Mihaela, Jurdana; Formaggio, Elena; Marina, Sciancalepore; Fumagalli, Guido Francesco; Zoran, Grubic; Fabio, Ruzzier; Paola, Lorenzon
Titolo:
Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro.
Anno:
2008
Tipologia prodotto:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Lingua:
Inglese
Formato:
A Stampa
Referee:
No
Nome rivista:
AMERICAN JOURNAL OF PHYSIOLOGY. CELL PHYSIOLOGY
ISSN Rivista:
0363-6143
N° Volume:
294
Intervallo pagine:
C66-C73
Note:
The aim of this study was to elucidate the mechanisms responsible for the effects of innervation on the maturation of excitation-contraction coupling apparatus in human skeletal muscle. For this purpose, we compared the establishment of the excitation-contraction coupling mechanism in myotubes differentiated in four different experimental paradigms: 1) aneurally cultured, 2) cocultured with fetal rat spinal cord explants, 3) aneurally cultured in medium conditioned by cocultures, and 4) aneurally cultured in medium supplemented with purified recombinant chick neural agrin. Ca(2+) imaging indicated that coculturing human muscle cells with rat spinal cord explants increased the fraction of cells showing a functional excitation-contraction coupling mechanism. The effect of spinal cord explants was mimicked by treatment with medium conditioned by cocultures or by addition of 1 nM of recombinant neural agrin to the medium. The treatment with neural agrin increased the number of human muscle cells in which functional ryanodine receptors (RyRs) and dihydropyridine-sensitive L-type Ca(2+) channels were detectable. Our data are consistent with the hypothesis that agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and that this effect is due to modulation of both RyRs and L-type Ca(2+) channels. Thus, a novel role for neural agrin in skeletal muscle maturation is proposed.
Id prodotto:
40098
Handle IRIS:
11562/315060
depositato il:
8 agosto 2008
ultima modifica:
1 dicembre 2022
Citazione bibliografica:
Elena, Bandi; Marko, Jevsek; Tomaz, Mars; Mihaela, Jurdana; Formaggio, Elena; Marina, Sciancalepore; Fumagalli, Guido Francesco; Zoran, Grubic; Fabio, Ruzzier; Paola, Lorenzon, Neural agrin controls maturation of the excitation-contraction coupling mechanism in human myotubes developing in vitro. «AMERICAN JOURNAL OF PHYSIOLOGY. CELL PHYSIOLOGY» , vol. 2942008pp. C66-C73

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Titolo Dipartimento Responsabili
Ruolo dell'attività e di fattori trofici nel controllo dell'espressione e della localizzazione subcellulare di canali al potassio a bassa conduttanza attivati dal calcio (SK3) nel muscolo scheletrico e in cellule nervose Dipartimento Ingegneria per la medicina di innovazione Guido Francesco Fumagalli
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