Publications

Authors:

Calderan, Laura; Vecchio, L; Boschi, Federico; Cerpelloni, Marzia; Franceschi, Antonia; Perbellini, Luigi; Sbarbati, Andrea; Osculati, Francesco

Title:

An in vivo and ex-vivo study of biodistribution and accumulation of Quantum Dots

Year:

2009

Type of item:

Contributo in atti di convegno

Tipologia ANVUR:

Contributo in Atti di convegno

Language:

Inglese

Format:

A Stampa

Congresso:

6th KEY SYMPOSIUM Nanomedicine

Place:

Stockholm, Sweden

Period:

September 9 - 11, 2009

Page numbers:

1-1

Keyword:

nanomedicine; in vivo biodistribution; central nervous system; immunitary system

Short description of contents:

In the present study we investigate the impact of nanoparticles in an in vivo and ex-vivo system. At this aim Balb-C female mice are treated with Quantum Dots (QDs) (1:5 dilutions of commercial solution 2 µM /0.01ml/g mouse, i.v) and sacrificed 3h, 24h and one week after injection (n=3 treated and n=3 control mice for every time point). Quantitative and qualitative bio-distribution of such nanoparticles is achieved by optical imaging and electron microscopy analysis. Optical data, acquired by a VivoVision Systems, IVIS®200 Series, Xenogen, show accumulation of QDs in anatomical region corresponding to brain, liver, spleen and lung. These results are supported by electron microscopy studies. After surgically extraction, the organs are fixed in 4% PF and embedded in LRW resin. Sections from every sample are collected on carbon coated nickel grid and observed with Philips Morgagni electron microscope equipped with a 40-50 µm objective aperture and operating at 80 kV. QDs takeover is not identify with membrane receptor system. Accumulation of such nanoparticles is detected in several cell types of those target organs. In particular 3h after injection QDs are localized in mitochondria, on the smooth endoplasmatic reticulum and in the lysosomes. Nuclei as well are labelled on dense chromatin and in the interchromatin space. Statistical analysis evidence a significant percentage of cells affected by QDs accumulation. The kinetics of cadmium, selenium and tellurium, present in the QDs and detected in different organs after their mineralization, was studied by Inductively Coupled Plasma/Mass Spectrometer (ICP-MS) analysis. The results validate the QDs storage in tissues.

Product ID:

51299

Handle IRIS:

11562/334047

Deposited On:

September 14, 2009

Last Modified:

November 14, 2022

Bibliographic citation:

Calderan, Laura; Vecchio, L; Boschi, Federico; Cerpelloni, Marzia; Franceschi, Antonia; Perbellini, Luigi; Sbarbati, Andrea; Osculati, Francesco, An in vivo and ex-vivo study of biodistribution and accumulation of Quantum Dots  in An in vivo and ex-vivo study of biodistribution and accumulation of Quantum Dots , Proceedings of "6th KEY SYMPOSIUM Nanomedicine" , Stockholm, Sweden , September 9 - 11, 2009 , 2009 , pp. 1-1

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