Publications

Authors:

Vezzalini, Marzia; Aletta, Jm; Beghelli, Stefania; Moratti, Elisabetta; DELLA PERUTA, Marco; Mafficini, Andrea; Mojica, Wd; Mombello, Aldo; Scarpa, Aldo; Sorio, Claudio

Title:

Immunohistochemical detection of arginine methylated proteins (MeRP) in archival tissues

Year:

2010

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

Sì

Name of journal:

Histopathology

ISSN of journal:

0309-0167

N° Volume:

57

Number or Folder:

5

Page numbers:

725-733

Keyword:

cancer; methylarginine; normal tissue; PRMT; immunohistochemistry

Short description of contents:

Aims: To (i) determine whether methylarginine-specific antibodies can be employed for standard immunohistochemical analysis of paraffin-embedded tissues, (ii) analyse methylarginine expression in normal and neoplastic tissues and (iii) correlate methylarginine expression with that of protein arginine methyltransferase (PRMT1), the predominant cellular arginine methyltransferase. Methods and results: Immunohistochemistry of normal and cancer tissues was performed utilizing three commercial polyclonal antibodies: anti-methylarginine-specific antibody (anti-mRG) raised against a methylarginine peptide, Control antibody (anti-RG), a control antiserum raised against a corresponding arginine peptide without any methylated residues and anti-PRMT1. Nuclear and/or cytoplasmic methylarginine expression was detected in all keratinized and non-keratinized epithelia. A preliminary survey of a series of thyroid, pancreatic, colonic and gastric cancers identified a different pattern of methylarginine expression in comparison with normal tissue. A correlation between methylarginine staining and PRMT1 expression was found in all normal and cancer tissues analysed. Conclusion: Methylarginine-specific antibodies are capable of recognizing methylarginine proteins (MeRP) in paraffin-embedded tissues. Methylarginine proteins are expressed widely and show differences in subcellular localization in various organs and neoplastic conditions. The efficient detection of methylproteins by standard immunohistochemistry provides a new tool to investigate the role of methylarginine proteins (MeRP) in biological processes including carcinogenesis.

Product ID:

58258

Handle IRIS:

11562/346358

Deposited On:

November 22, 2010

Last Modified:

November 15, 2022

Bibliographic citation:

Vezzalini, Marzia; Aletta, Jm; Beghelli, Stefania; Moratti, Elisabetta; DELLA PERUTA, Marco; Mafficini, Andrea; Mojica, Wd; Mombello, Aldo; Scarpa, Aldo; Sorio, Claudio, Immunohistochemical detection of arginine methylated proteins (MeRP) in archival tissues «Histopathology» , vol. 57 , n. 5 , 2010 , pp. 725-733

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