Publications

Authors:

Nicolis, Elena; I., Lampronti; M. C., Dechecchi; M., Borgatti; Tamanini, Anna; Bezzerri, Valentino; N., Bianchi; M., Mazzon; I., Mancini; Giri, Mariagrazia; P., Rizzotti; R., Gambari; Cabrini, Giulio

Title:

Modulation of expression of IL-8 gene in bronchial epithelial cells by 5-methoxypsoralen.

Year:

2009

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Referee:

Sì

Name of journal:

International Immunopharmacology

ISSN of journal:

1567-5769

N° Volume:

9

Number or Folder:

12

:

Elsevier

Page numbers:

1411-1422

Keyword:

Argemone, Bronchi; pathology, Cell Line, Cell Proliferation, Cystic Fibrosis; drug therapy/genetics/immunology, Epithelial Cells; immunology/metabolism, Gene Expression Regulation, Humans, Interleukin-8; genetics/immunology/metabolism, Methoxsalen; administration /&/ dosage/analogs /&/ derivatives, Neutrophils; drug effects/pathology, Photosensitizing Agents; administration /&/ dosage, Plant Extracts, Pseudomonas aeruginosa; immunology/pathogenicity, Vernonia

Short description of contents:

Persistent recruitment of neutrophils in the bronchi of cystic fibrosis patients contributes to airway tissue damage, suggesting the importance of intervening on the expression of the neutrophil chemokine IL-8. Extracts from plants have been investigated to select components able to reduce IL-8 expression in bronchial epithelial cells challenged with Pseudomonas aeruginosa. Extracts and purified components have been added to cells 24 h before pro-inflammatory challenge with P. aeruginosa and IL-8 transcription was quantified in the IB3-1 CF cells in vitro. P. aeruginosa-dependent IL-8 mRNA induction was increased by Argemone mexicana and Vernonia anthelmintica whereas no significant modification of transcription was observed with Aphanamixis polystachya, Lagerstroemia speciosa and Hemidesmus indicus. Finally, inhibition of IL-8 was observed with Polyalthia longifolia (IC50=200 microg/ml) and Aegle marmelos (IC50=20 microg/ml). Compounds from A. marmelos were isolated and identified by GC-MS. No significant effect was observed with butyl-p-tolyl sulphate, whereas the inhibition obtained with 6-methyl-4-chromanone concentration was accompanied by an anti-proliferative effect. On the contrary, 5-methoxypsoralen resulted in IL-8 inhibition at 10 microM concentration, without effects on cell proliferation. In synthesis, 5-methoxypsoralen can be taken into consideration to investigate mechanisms of neutrophil chemotactic signalling and for its potential application in modulating the excessive CF lung inflammation.

Web page:

http://dx.doi.org/10.1016/j.intimp.2009.08.013

Product ID:

82832

Handle IRIS:

11562/386729

Deposited On:

September 8, 2014

Last Modified:

November 15, 2022

Bibliographic citation:

Nicolis, Elena; I., Lampronti; M. C., Dechecchi; M., Borgatti; Tamanini, Anna; Bezzerri, Valentino; N., Bianchi; M., Mazzon; I., Mancini; Giri, Mariagrazia; P., Rizzotti; R., Gambari; Cabrini, Giulio, Modulation of expression of IL-8 gene in bronchial epithelial cells by 5-methoxypsoralen. «International Immunopharmacology» , vol. 9 , n. 12 , 2009 , pp. 1411-1422

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