Publications

Authors:

Paleari, R; Montagnana, Martina; Danese, Elisa; Tozzi, M; Guidi, Giancesare; Mosca, M.

Title:

Determinazione della variabilità biologica dell’emoglobina A2. [Determination of biological variation of hemoglobin A 2]

Year:

2011

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Italiano

Format:

A Stampa

Referee:

Sì

Name of journal:

Biochimica Clinica

ISSN of journal:

0393-0564

N° Volume:

35

Number or Folder:

6

Page numbers:

458-460

Keyword:

Biological variation; hemoglobin A2; HbA2.

Short description of contents:

We present an experimental report aimed to evaluate the biological variation of hemoglobin A2 (HbA2), a minor hemoglobin component in post-natal life, accounting for 2.5%-3.5% of the total hemoglobin in red cells, which is very relevant for the laboratory diagnosis of thalassemic syndromes. We took five blood specimens from 17 apparently healthy subjects (9 men and 8 women, ages 26-52 years) on the same day, every two weeks for two months. Samples were stored at -80 °C until analysis andassayed in duplicate by Bio-Rad Variant II analyzer. Data were analyzed by the ANOVA. There were no differences in HbA2 values between men and women. HbA2 exhibited marked individuality: within- (CVI) and between-subject (CVG) biological variation were 0.7% and 7.7%, respectively. Desirable analytical goals derived from biological variation for imprecision (0.5 CVI), bias [0.25 (CVI 2 + CVG 2)1/2] and total error [1.65 (0.5 CVI) + 0.25 (CVI 2 + CVG 2)1/2] were 0.4%, 1.9%, and 3.1%, respectively. In conclusion, this is the first evidence that HbA2, as well as total hemoglobin, is under a strict homeostatic control. Our data also show that stringent analytical goals are needed for the clinical application of HbA2 measurements.

Product ID:

73922

Handle IRIS:

11562/474959

Deposited On:

November 8, 2012

Last Modified:

November 12, 2022

Bibliographic citation:

Paleari, R; Montagnana, Martina; Danese, Elisa; Tozzi, M; Guidi, Giancesare; Mosca, M., Determinazione della variabilità biologica dell’emoglobina A2. [Determination of biological variation of hemoglobin A 2] «Biochimica Clinica» , vol. 35 , n. 6 , 2011 , pp. 458-460

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