Pubblicazioni

Autori:

Brandi, Jessica; DALLA POZZA, Elisa; Dando, Ilaria; Biondani, Giulia; V., Elliott; E., Costello; Palmieri, Marta; Cecconi, Daniela

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Titolo:

SHOTGUN PROTEOMIC ANALYSIS OF PANCREATIC CANCER STEM CELLS

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Anno:

2014

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Tipologia prodotto:

Poster

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Tipologia ANVUR:

Poster

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Lingua:

Inglese

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Titolo del Convegno:

13th Human Proteome Organization World Congress

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Luogo:

Madrid

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Periodo:

5-8/10/2014

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Intervallo pagine:

1-1

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Parole chiave:

Shotgun Proteomics

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Breve descrizione dei contenuti:

Introduction and objectives Pancreatic adenocarcinoma (PDAC) is initiated and sustained by a subpopulation of cells defined as cancer stem cells (PCSCs). PCSCs are responsible for pancreatic tumorigenesis, tumour progression, and resistance to chemotherapeutic treatment.The aim of this work is the identification of new biomarkers or diagnostic/therapeutic targets of PDAC, through a proteomic comparison of a PDAC cell line and the derived PCSCs. Methods In this study, we performed an iTRAQ 8-plex/2D-LC-MS/MS analysis to compare the secretomes and whole proteomes of Panc1 and PCSCs cell lines. Results and Discussion The iTRAQ approach allowed us to identify 1146 proteins. Out of these, 111 proteins were found have higher abundance in the conditioned media as compared to the whole cell lysate of Panc1 and PCSCs cells. By using secretion prediction tools, we have found that 25% of the 111 proteins is exported by the classical secretory pathways, while the 75% is secreted by non-classical pathways. Then we performed a quantitative comparison of Panc1 and PCSCs secretomes, and whole proteome, identifying 71 and 162 differentially expressed proteins respectively. The global implications of secreted and intracellular differentially regulated proteins were elucidated by the Ingenuity Pathway Analysis (IPA). The differentially expressed proteins are involved in cell proliferation, invasion and contribute to epithelial-mesenchymal transition (EMT). Conclusions Our results indicate that iTRAQ technology is a promising way to investigate the PCSC biological model and to find useful biomarkers for PDAC.

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Id prodotto:

84750

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Handle IRIS:

11562/830365

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depositato il:

19 gennaio 2015

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ultima modifica:

4 novembre 2022

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Citazione bibliografica:

Brandi, Jessica; DALLA POZZA, Elisa; Dando, Ilaria; Biondani, Giulia; V., Elliott; E., Costello; Palmieri, Marta; Cecconi, Daniela, SHOTGUN PROTEOMIC ANALYSIS OF PANCREATIC CANCER STEM CELLS , Atti di "13th Human Proteome Organization World Congress" , Madrid , 5-8/10/2014 , 2014 , pp. 1-1

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