Pubblicazioni

Autori:

De Souza Lima Oliveira, Gabriel; Lippi, Giuseppe; Salvagno, GIAN LUCA; Gaino, Stefania; Poli, Giovanni; Gelati, Matteo; Picheth, Geraldo; Guidi, Giancesare

Titolo:

Venous stasis and whole blood platelet aggregometry: a question of data reliability and patient safety

Anno:

2015

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Blood Coagulation and Fibrinolysis

ISSN Rivista:

0957-5235

N° Volume:

26

Numero o Fascicolo:

6

Intervallo pagine:

665-668

Parole chiave:

platelet function, aggregometry, laboratory hematology

Breve descrizione dei contenuti:

The assessment of platelet function by multiple electrode aggregometry (MEA) (Multiplate, Roche Diagnostics GmbH) is common in laboratory hematology. As regards the ISO 15189:2012 international standard, appropriate use of laboratory equipment requires appropriate pre-examination activities (e.g., blood collection). Venous stasis can influence several blood analytes, but the tourniquet time is rarely regarded as a source of variability. Aim of the present study was to evaluate the impact of venous stasis on platelet function by MEA. A total of 6 ml of blood was collected from 20 volunteers into two 3.0 ml Hirudin vacuum tube (Roche Diagnostics GmbH), and subjected to two procedures: procedure 1 (no stasis) - collection after localization of forearm vein by subcutaneous tissue transilluminator device without tourniquet; procedure 2 (stasis) - collection after localization of vein by prior 60 s tourniquet application. Samples were processed on Multiplate, for: ADP-test (without prostaglandin E1), ADP HS-test (with prostaglandin E1), ASPI-test, COL-test, RISTO H-test (high concentration, 0.77 mg/ml), RISTO L-test (low concentration, 0.20 mg/ml), and TRAP-test. The significance of the differences between samples was assessed by Wilcoxon ranked-pairs test. Surprisingly, the results of ADP HS-test, ASPI-test, COL-test, and RISTO H-test appeared unbiased by venous stasis. RISTO L-test, ADP-test, and TRAP-test were significantly biased; the mean percent difference between stasis and no stasis were -7.2% (P = 0.040), -28.4% (P = 0.015), and 1.1% (P = 0.031), respectively. In conclusion, the tourniquet should be avoided when assessing platelet function by MEA.

Id prodotto:

88338

Handle IRIS:

11562/927581

ultima modifica:

15 novembre 2022

Citazione bibliografica:

De Souza Lima Oliveira, Gabriel; Lippi, Giuseppe; Salvagno, GIAN LUCA; Gaino, Stefania; Poli, Giovanni; Gelati, Matteo; Picheth, Geraldo; Guidi, Giancesare, Venous stasis and whole blood platelet aggregometry: a question of data reliability and patient safety «Blood Coagulation and Fibrinolysis» , vol. 26 , n. 6 , 2015 , pp. 665-668

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