Publications

Secretome protein signature of human pancreatic cancer stem-like cells  (2016)

Authors:
Brandi, Jessica; Pozza, Elisa Dalla; Dando, Ilaria; Biondani, Giulia; Robotti, Elisa; Jenkins, Rosalind; Elliott, Victoria; Park, Kevin; Marengo, Emilio; Costello, Eithne; Scarpa, Aldo; Palmieri, Marta; Cecconi, Daniela
Title:
Secretome protein signature of human pancreatic cancer stem-like cells
Year:
2016
Type of item:
Articolo in Rivista
Tipologia ANVUR:
Articolo su rivista
Language:
Inglese
Referee:
Name of journal:
JOURNAL OF PROTEOMICS
ISSN of journal:
1874-3919
N° Volume:
136
Page numbers:
1-12
Keyword:
Pancreatic cancer; Secretome; stem-cell
Short description of contents:
Emerging research has demonstrated that pancreatic ductal adenocarcinoma (PDAC) contains a sub-population of cancer stem cells (CSCs) characterized by self-renewal, anchorage-independent-growth, long-term proliferation and chemoresistance. The secretome analysis of pancreatic CSCs has not yet been performed, although it may provide insight into tumour/microenvironment interactions and intracellular processes, as well as to identify potential biomarkers. To characterize the secreted proteins of pancreatic CSCs, we performed an iTRAQ-based proteomic analysis to compare the secretomes of Panc-1 cancer stem-like cells (Panc1 CSCs) and parental cell line. A total of 72 proteins were found up-/down-regulated in the conditioned medium of Panc-1 CSCs. The pathway analysis revealed modulation of vital physiological pathways including glycolysis, gluconeogenesis and pentose phosphate. Through ELISA immunoassays we analysed the presence of the three proteins most highly secreted by Panc-1 CSCs (ceruloplasmin, galectin-3, and MARCKS) in sera of PDAC patient. ROC curve analysis suggests ceruloplasmin as promising marker for patients negative for CA19-9. Overall, our study provides a systemic secretome analysis of pancreatic CSCs revealing a number of secreted proteins which participate in pathological conditions including cancer differentiation, invasion and metastasis. They may serve as a valuable pool of proteins from which biomarkers and therapeutic targets can be identified.
Web page:
http://www.sciencedirect.com/science/article/pii/S1874391916300203
Product ID:
90646
Handle IRIS:
11562/935533
Last Modified:
May 22, 2017
Bibliographic citation:
Brandi, Jessica; Pozza, Elisa Dalla; Dando, Ilaria; Biondani, Giulia; Robotti, Elisa; Jenkins, Rosalind; Elliott, Victoria; Park, Kevin; Marengo, Emilio; Costello, Eithne; Scarpa, Aldo; Palmieri, Marta; Cecconi, Daniela, Secretome protein signature of human pancreatic cancer stem-like cells «JOURNAL OF PROTEOMICS» , vol. 1362016pp. 1-12

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Ricerca di marcatori molecolari proteici nel tumore pancreatico tramite analisi proteomiche di cellule staminali tumorali Department Biotecnologie Daniela Cecconi, Marta Palmieri
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