Publications

Authors:

Fabbri, Enrica; Tamanini, Anna; Jakova, Tiziana; Gasparello, Jessica; Manicardi, Alex; Corradini, Roberto; Sabbioni, Giuseppe; Finotti, Alessia; Borgatti, Monica; Lampronti, Ilaria; Munari, Silvia; Dechecchi, Mariacristina; Cabrini, Giulio; Gambari, Roberto

Title:

A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells

Year:

2017

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

MOLECULES

ISSN of journal:

1420-3049

N° Volume:

23

Number or Folder:

1

Page numbers:

71-71

Keyword:

CFTR; cystic fibrosis; delivery; miR-145; miRNA targeting; microRNAs; peptide nucleic acids

Short description of contents:

Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular in the last years their use for targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to the repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective in cystic fibrosis, are of great importance in the development of new type of treatments. In this paper we propose the use of an anti-miR PNA for targeting miR-145, a microRNA reported to suppress CFTR expression. Octaarginine-anti-miR PNA conjugates were delivered to Calu-3 cells, exerting sequence dependent targeting of miR-145-5p. This allowed to enhance expression of the miR-145 regulated CFTR gene, analyzed at mRNA (RT-qPCR, Reverse Transcription quantitative Polymerase Chain Reaction) and CFTR protein (Western blotting) level.

Product ID:

99893

Handle IRIS:

11562/971787

Last Modified:

November 28, 2022

Bibliographic citation:

Fabbri, Enrica; Tamanini, Anna; Jakova, Tiziana; Gasparello, Jessica; Manicardi, Alex; Corradini, Roberto; Sabbioni, Giuseppe; Finotti, Alessia; Borgatti, Monica; Lampronti, Ilaria; Munari, Silvia; Dechecchi, Mariacristina; Cabrini, Giulio; Gambari, Roberto, A Peptide Nucleic Acid against MicroRNA miR-145-5p Enhances the Expression of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Calu-3 Cells «MOLECULES» , vol. 23 , n. 1 , 2017 , pp. 71-71

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