Publications

Authors:

Benati, Marco; Danese, Elisa; Montagnana, Martina; Paviati, Elisa; Maria Minicozzi, Anna; Gusella, Milena; Pasini, Felice; Lippi, Giuseppe

Title:

Telomere shortening and PCDH10 promoter methylation in colorectal cancer mucosae

Year:

2019

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Biochimica Clinica

ISSN of journal:

0393-0564

N° Volume:

43

Number or Folder:

3

Page numbers:

279-284

Keyword:

Telomere shortening, PCDH10, promoter, methylation, colorectal cancer

Short description of contents:

Background: telomerase activity and telomere length (TL) have important implications in several human diseases.Telomere shortening is associated with colorectal carcinogenesis. Recent studies also showed that protocadherin 10(PCDH10) plays a critical role in cancer cell growth, by negatively regulating telomerase activity. PCDH10isfrequently downregulated by promoter DNA methylation. The aim of this study was to investigate whether PCDH10promoter methylation was associated with TL in colorectal cancer (CRC). Methods: DNA was extracted from 35 CRC and 35 adjacent normal tissues with Gentra Purgene Kit (Qiagen, Hilden,Germany). A quantitative methylation-specific PCR (MSP) based method was used to analyze a selected CpG site inPCDH10promoter. TL was evaluated with qPCR and expressed as telomere to single copy gene (T/S) ratio.Differences were assessed with Mann-Whitney test or Wilcoxon signed-ranks test when appropriate, whilstcorrelation analyses were performed with Spearman’s test. Diagnostic performance was calculated with receiveroperating characteristics (ROC) curve analysis. The level of statistical significance was set at p <0.05. Results: we found that TL was significantly lower in CRC than in adjacent non-cancerous tissues (p=0.0005). Thearea under the ROC curve (AUC) for TL was 0.759 (95% Confidence Interval: 0.643-0.875, p=0.0002). AberrantPCDH10promoter methylation was detected in 100% of CRC tissues but in none of paired non-cancerous tissues.The median methylation rate in CRC tissues was 55.7% (range: 6.1-97.8%). TL was negatively correlated withPCDH10promoter methylation (r=-0.42, p=0.0002). Conclusions: these results suggest a pivotal role of telomere shortening and PCDH10methylation in CRC tissues.TL may be seen as a potential biomarker in CRC diagnostics.

Product ID:

110060

Handle IRIS:

11562/999514

Last Modified:

November 15, 2022

Bibliographic citation:

Benati, Marco; Danese, Elisa; Montagnana, Martina; Paviati, Elisa; Maria Minicozzi, Anna; Gusella, Milena; Pasini, Felice; Lippi, Giuseppe, Telomere shortening and PCDH10 promoter methylation in colorectal cancer mucosae «Biochimica Clinica» , vol. 43 , n. 3 , 2019 , pp. 279-284

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo