Publications

Authors:

Romanelli, Maria; Lorenzi, Pamela; Diani, Erica; Filippello, Agnese Rita Filippa Tindara; Avesani, F; Morandi, Carlo

Title:

Transcriptional regulation of the human Raver2 ribonucleoprotein gene.

Year:

2012

Type of item:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Language:

Inglese

Format:

A Stampa

Referee:

Sì

Name of journal:

Gene

ISSN of journal:

0378-1119

N° Volume:

493

:

Elsevier

Page numbers:

243-252

Keyword:

ribonucleoproteins; gene expression; polypyrimidine-tract binding protein

Short description of contents:

Raver2 is a putative modulator of the activity of the polypyrimidine-tract binding protein (PTB), one of the most intensively studied splicing repressors. Little is known about Raver2 expression, and all current data is from mice where it shows tissue specificity. In the present study, by comparing Raver2 transcript expression in human and mouse tissues, we found that human Raver2 is ubiquitously expressed in adult tissues. In order to investigate human Raver2 transcription regulation, we identified and characterized a putative promoter region in a 1000bp region upstream of the transcription starting site of the gene. Dual luciferase reporter assays demonstrated that this region had promoter activity conferred by the first 160bp. By mutagenic analyses of putative cis-acting regulatory sequences, we identified an individual site that decreased the promoter activity by up to 40% when mutated. Together, our results suggest that regulation of human Raver2 expression involves TATA-less transcriptional activity.

Web page:

http://www.ncbi.nlm.nih.gov/pubmed/22146317

Product ID:

66006

Handle IRIS:

11562/390267

Deposited On:

February 21, 2012

Last Modified:

November 11, 2022

Bibliographic citation:

Romanelli, Maria; Lorenzi, Pamela; Diani, Erica; Filippello, Agnese Rita Filippa Tindara; Avesani, F; Morandi, Carlo, Transcriptional regulation of the human Raver2 ribonucleoprotein gene. «Gene» , vol. 493 , 2012 , pp. 243-252

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