Publications

Proteins released from Pseudomonas aeruginosa (Pa) referent and clinical strains under microaerobiosis and their effects on cystic fibrosis airways  (2008)

Authors:
Cigana C.; Mauri P.; Di Silvestre D.; Bergamini G.; Bragonzi A.; Assael B.M.; Sorio C.; Melotti P.
Title:
Proteins released from Pseudomonas aeruginosa (Pa) referent and clinical strains under microaerobiosis and their effects on cystic fibrosis airways
Year:
2008
Type of item:
Contributo in atti di convegno
Tipologia ANVUR:
Contributo in Atti di convegno
Language:
Inglese
Format:
A Stampa
Name of journal:
Journal of Cystic Fibrosis
ISSN of journal:
1569-1993
N° Volume:
7
Number or Folder:
suppl 2
Congresso:
31st European Cystic Fibrosis Conference,
Place:
Prague, Czech Republic
Period:
11-14 June 2008
:
Elsevier
Publisher:
Elsevier
Page numbers:
S4-S4
Keyword:
Pseudomonas Aeruginosa, cystic fibrosis, inflammation
Short description of contents:
Little is known about changes in protein secretion in Pa laboratory and clinical strains induced by oxygen limitation and their effects on epithelial cells. Here, we demonstrated that conditioned medium from PAO1 and clinical strain AA2 under aerobiosis induced a statistically significant increase of about 2.9 and 4.9 times, respectively of IL-8 and TNF-_ mRNA, in CF airway epithelial cells. This induction was reduced of about 25% when strains were grown under microaerobiosis, suggesting that adaptation to microaerobiosis reduces Pa pathogenicity. Two-dimensional capillary chromatographytandem mass spectrometry (MudPIT) identified 75 polypeptides released by PAO1 and 198 by AA2 in aerobiosis. Differential expression was observed under microaerobiosis when compared to aerobiosis with 6 proteins up-regulated in PAO1 and 7 proteins upregulated in AA2; 4 proteins were downregulated in AA2. 7 proteases were released from AA2 while only 1 from PAO1 with strong down modulation of alkaline metalloprotease in microaerobiosis by AA2 as confirmed by proteolytic activity assay. Identification of virulence factors might be facilitated by this combined approach suggesting molecular targets for pharmacological intervention. Supported by: Italian Cystic Fibrosis Research Foundation FFCgrant17/2006; Comitato Vicenza-Associazione Veneta Lotta contro la Fibrosi Cistica; Azienda Ospedaliera Verona, Italy. Equal contribution: C Cigana and P Mauri.
Product ID:
55704
Handle IRIS:
11562/320267
Deposited On:
April 9, 2010
Last Modified:
November 2, 2016
Bibliographic citation:
Cigana C.; Mauri P.; Di Silvestre D.; Bergamini G.; Bragonzi A.; Assael B.M.; Sorio C.; Melotti P., Proteins released from Pseudomonas aeruginosa (Pa) referent and clinical strains under microaerobiosis and their effects on cystic fibrosis airways in «Journal of Cystic Fibrosis» vol. 7 n. suppl 2 ElsevierProceedings of "31st European Cystic Fibrosis Conference," , Prague, Czech Republic , 11-14 June 2008 , 2008pp. S4-S4

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