Publications

Comparison of Tax-1 and Tax-2B posttranslational modifications using specific lysine mutants in relation to activation of NF-B and intracellular localization  (2011)

Authors:
Turci M, Di Gennaro G, Cotena A, Marin O, Avesani F, Cremonese G, Diani E, Romanelli MG, Bertazzoni U.
Title:
Comparison of Tax-1 and Tax-2B posttranslational modifications using specific lysine mutants in relation to activation of NF-B and intracellular localization
Year:
2011
Type of item:
Contributo in atti di convegno
Tipologia ANVUR:
Contributo in Atti di convegno
Nations of authors:
ITALIA
Language:
Inglese
Format:
Elettronico
Name of journal:
Retrovirology
ISSN of journal:
1742-4690
N° Volume:
8
Congresso:
15th International Conference on Human Retroviruses: HTLV and Related Viruses
Place:
Leuven and Gembloux, Belgium
Period:
5-8 June 2011
Page numbers:
A143-A143
Keyword:
HTLV,Tax,NF-kB
Short description of contents:
ost-translational modifications of HTLV-1 and HTLV-2 Tax-1 and Tax-2 proteins have been shown to play a critical role in their cellular localization, transactivation and protein interactions. Five of ten lysine residues were found to be major targets for Tax-1 modifications: Lys189(K4); Lys197(K5), Lys263(K6), Lys280(K7) and Lys284(K8), are essential for ubiquitination, while sumoylation takes place on Lys280 (K7) and Lys284(K8). Tax-2 contains four additional lysine residues, namely at position Lys100(K2i), Lys149(K3i), Lys185(K3ii), and Lys356(K10i). Very few studies have been so far performed on Tax-2 lysine mutants. We have previously demonstrated that Tax-2B is ubiquitinated and sumoylated similarly to Tax-1. To identify the Tax-2 lysine residues which are directly involved in post-translational modifications, we have constructed a series of Tax-2B mutants with substitutions of lysine (K) residues by arginines (R) and analyzed them for NF-kB and CREB/ATF transactivation, intracellular distribution and extent of ubiquitination and sumoylation. We have found that Tax-2 K7-8R mutant, contrary to its Tax-1 homologue, is only partially affected in its capacity to transactivate NF-κB pathway, is regularly sumoylated and presents formation of nuclear bodies by confocal analysis. However, Tax-2 mutants with extended (K3ii-8R) and/or total (K1-10iR) mutation rate were severely affected for NF-kB transactivation and sumoylation. By comparing Tax-2 WT with mutants K7-8R and K3ii-8R, we observed that the reduction of NF-κB activity is correlated to a parallel decrease in sumoylation. These results suggest that the target for Tax-2 ubiquitination and sumoylation differs from that described for Tax-1.
Web page:
http://www.retrovirology.com/content/8/S1/A143
Product ID:
66086
Handle IRIS:
11562/390452
Deposited On:
February 22, 2012
Last Modified:
February 22, 2012
Bibliographic citation:
Turci M, Di Gennaro G, Cotena A, Marin O, Avesani F, Cremonese G, Diani E, Romanelli MG, Bertazzoni U., Comparison of Tax-1 and Tax-2B posttranslational modifications using specific lysine mutants in relation to activation of NF-B and intracellular localization in «Retrovirology» vol. 8Proceedings of "15th International Conference on Human Retroviruses: HTLV and Related Viruses" , Leuven and Gembloux, Belgium , 5-8 June 2011 , 2011pp. A143-A143

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