Publications

IKKepsilon involvement in Tax-mediated activation of INF pathway  (2011)

Authors:
Cremonese G, Avesani F, Diani E, Turci M, Di Gennaro G, Bertazzoni U, Romanelli MG
Title:
IKKepsilon involvement in Tax-mediated activation of INF pathway
Year:
2011
Type of item:
Contributo in atti di convegno
Tipologia ANVUR:
Contributo in Atti di convegno
Nations of authors:
ITALIA
Language:
Inglese
Name of journal:
Retrovirology
ISSN of journal:
1742-4690
N° Volume:
8
Congresso:
15th International Conference on Human Retroviruses: HTLV and Related Viruses
Place:
Leuven and Gembloux, Belgium
Period:
5-8 June 2011
Page numbers:
A137-A137
Keyword:
HTLV,Tax,interferon
Short description of contents:
HTLV-1 Tax de-regulates several cellular signaling pathways leading to cell transformation by altering gene expression, intracellular protein distribution and cell proliferation. Tax-1 induces persistent activation of several transcriptional factors and signal transduction pathways, including NF-κB and CREB/ATF. It is known that Tax-1 constitutively activates TAK1 (transforming growth factor-β-activated kinase 1) and modifies the interferon (INF) regulatory signals by controlling the expression of INF transcription factors 3 (INF3) and INF4. We have recently reported that HTLV-1 and HTLV-2 Tax proteins interact with TAK1-binding protein 2 (TAB2) of the NF-κB pathway and that both Tax proteins transactivate NF-κB promoters [1]. TAB2 functions as an adaptor protein to recruit TAK1 to TRAF2 (TNF-α receptor-associated factor) in TNF-α signaling pathways. In the present study we have investigated Tax-1 and Tax-2 role in modifying INF and NF-κB activation through the recruitment of IKKepsilon, an IκB kinase homologue involved in NF-κB and INF3 signaling pathways. By co-immunoprecipitation experiments, we have found that both IKKepsilon and Tax-1, but not Tax-2, are present in protein complexes in transfected cells. IKKepsilon and Tax-1 or Tax-2 role in the activation of INF responsive elements or NF-κB containing promoters have been analyzed after transfecting the protein genes in 293T cells and measuring the effect by luciferase assay. Co-expression of Tax-1 and IKKepsilon resulted in an increased IRF activation mediated by IKKepsilon. Interaction of IKKepsilon with Tax-1 and Tax-2 and their possible effects in the de-regulation of the IRF3 pathways will be discussed.
Web page:
http://www.retrovirology.com/content/8/S1/A137
Product ID:
66088
Handle IRIS:
11562/390453
Deposited On:
February 22, 2012
Last Modified:
February 22, 2012
Bibliographic citation:
Cremonese G, Avesani F, Diani E, Turci M, Di Gennaro G, Bertazzoni U, Romanelli MG, IKKepsilon involvement in Tax-mediated activation of INF pathway in «Retrovirology» vol. 8Proceedings of "15th International Conference on Human Retroviruses: HTLV and Related Viruses" , Leuven and Gembloux, Belgium , 5-8 June 2011 , 2011pp. A137-A137

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