Pubblicazioni

Autori:

Paleari, R; Montagnana, Martina; Danese, Elisa; Tozzi, M; Guidi, Giancesare; Mosca, M.

Titolo:

Determinazione della variabilità biologica dell’emoglobina A2. [Determination of biological variation of hemoglobin A 2]

Anno:

2011

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Italiano

Formato:

A Stampa

Referee:

Sì

Nome rivista:

Biochimica Clinica

ISSN Rivista:

0393-0564

N° Volume:

35

Numero o Fascicolo:

6

Intervallo pagine:

458-460

Parole chiave:

Biological variation; hemoglobin A2; HbA2.

Breve descrizione dei contenuti:

We present an experimental report aimed to evaluate the biological variation of hemoglobin A2 (HbA2), a minor hemoglobin component in post-natal life, accounting for 2.5%-3.5% of the total hemoglobin in red cells, which is very relevant for the laboratory diagnosis of thalassemic syndromes. We took five blood specimens from 17 apparently healthy subjects (9 men and 8 women, ages 26-52 years) on the same day, every two weeks for two months. Samples were stored at -80 °C until analysis andassayed in duplicate by Bio-Rad Variant II analyzer. Data were analyzed by the ANOVA. There were no differences in HbA2 values between men and women. HbA2 exhibited marked individuality: within- (CVI) and between-subject (CVG) biological variation were 0.7% and 7.7%, respectively. Desirable analytical goals derived from biological variation for imprecision (0.5 CVI), bias [0.25 (CVI 2 + CVG 2)1/2] and total error [1.65 (0.5 CVI) + 0.25 (CVI 2 + CVG 2)1/2] were 0.4%, 1.9%, and 3.1%, respectively. In conclusion, this is the first evidence that HbA2, as well as total hemoglobin, is under a strict homeostatic control. Our data also show that stringent analytical goals are needed for the clinical application of HbA2 measurements.

Id prodotto:

73922

Handle IRIS:

11562/474959

depositato il:

8 novembre 2012

ultima modifica:

12 novembre 2022

Citazione bibliografica:

Paleari, R; Montagnana, Martina; Danese, Elisa; Tozzi, M; Guidi, Giancesare; Mosca, M., Determinazione della variabilità biologica dell’emoglobina A2. [Determination of biological variation of hemoglobin A 2] «Biochimica Clinica» , vol. 35 , n. 6 , 2011 , pp. 458-460

Consulta la scheda completa presente nel repository istituzionale della Ricerca di Ateneo