“RNA metabolism-related genes expression in response to microenvironmental changes in myotubes differentiation and in COPD”.

Starting date
January 1, 2010
Duration (months)
36
Managers or local contacts
Morandi Carlo
Keyword
PTB, ribonucleoproteins, splicing,

This program represents the natural follow up of a long sought and well documented interest of the research unit towards PTB and Raver proteins, which interact with PTB as co-repressors. Deciphering the alternative splicing regulator factors involvement in the regulation of specific muscle exons will allow to the identification of muscle-specific modulators of gene expression during differentiation and adaptation to exercise. It may contribute to the elucidation of the complex molecular mechanisms and splicing-regulatory networks that regulate gene expression in skeletal muscle and identify molecular markers for muscle dysfunctions.
In particular, the project will investigate ribonucleoprotein expression pattern, localization and roles in alternative splicing events that occur in muscle specific transcripts during myotube differentiation and microenvironmental changes. Comparative assessment of ribonuceoproteins expression pattern in peripheral blood leukocytes in COPD will contribute to the knowledge of gene expression regulation machinery involved in the adaptation to physical activity.

Sponsors:

PRIN VALUTATO POSITIVAMENTE
Funds: requested
Syllabus: PRIN

Project participants

Erica Diani
Scholarship holder
Agnese Rita Filippa Tindara Filippello
Pamela Lorenzi
Technical-administrative staff
Publications
Title Authors Year
Transcriptional regulation of the human Raver2 ribonucleoprotein gene. Romanelli MG; Lorenzi P; Diani E; Filippello A; Avesani F; Morandi C. 2012
Molecular cloning and expression analyses of the RBM20 gene that is associated to familial dilated cardiomyopathy A. Filippello; P. Lorenzi; C. Morandi; M.G. Romanelli 2011
Functional characterization of the human ribonucleoprotein, PTB-binding 2/Raver2 promoter region. Lorenzi P.; Morandi C; Romanelli MG; 2008

Activities

Research facilities