Pubblicazioni

Autori:

Mariotto, Sara; de Gaspari, Piera; Jäger, Dominik; Hahn, Stefanie; Forni, Cindy; Saschenbrecker, Sandra; Lattwein, Erik; Dinoto, Alessandro; Ferrari, Sergio

Titolo:

A novel cell-based immunofluorescence assay for the detection of autoantibodies to myelin-associated glycoprotein

Anno:

2023

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

FRONTIERS IN NEUROLOGY

ISSN Rivista:

1664-2295

N° Volume:

14

Numero o Fascicolo:

1289810

Intervallo pagine:

1-4

Parole chiave:

HNK-1; IgM autoantibodies; MAG; cell-based assay; human natural killer-1; myelin-associated glycoprotein; neuropathy

Breve descrizione dei contenuti:

Peripheral neuropathy with antibodies to myelin-associated glycoprotein (MAG) is an autoimmune demyelinating disorder of the peripheral nervous system caused by pathogenic IgM recognizing the human natural killer-1 glycoepitope expressed on MAG. This study aimed to analyze the performance of a new indirect immunofluorescence cell-based assay (CBA, EUROIMMUN) for the detection of anti-MAG IgM. Antibody reactivity was determined in sera from 95 patients with clinical and neurophysiological evidence of anti-MAG-associated neuropathy and in control samples from 55 patients with other forms of peripheral neuropathy. Compared to the results of the gold standard method (ELISA, Buhlmann) and using samples at a dilution of 1:100, the CBA had a sensitivity of 98.9% and a specificity of 100% (PPV 100%, NPV 98.2%). In conclusion, the CBA allows the detection of antibodies to MAG using an easy and standardized technique, and it presents a sensitive and specific alternative to the more time-consuming ELISA. Larger studies are needed to address anti-MAG titer monitoring in parallel with clinical activity.

Pagina Web:

https://doi.org/10.3389/fneur.2023.1289810

Id prodotto:

136995

Handle IRIS:

11562/1116770

ultima modifica:

10 gennaio 2024

Citazione bibliografica:

Mariotto, Sara; de Gaspari, Piera; Jäger, Dominik; Hahn, Stefanie; Forni, Cindy; Saschenbrecker, Sandra; Lattwein, Erik; Dinoto, Alessandro; Ferrari, Sergio, A novel cell-based immunofluorescence assay for the detection of autoantibodies to myelin-associated glycoprotein «FRONTIERS IN NEUROLOGY» , vol. 14 , n. 1289810 , 2023 , pp. 1-4

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