Pubblicazioni

Autori:

Garbin, Ulisse; FRATTA PASINI, Anna Maria; Stranieri, Chiara; Cominacini, M; Pasini, Andrea; Manfro, Stefania; Lugoboni, F; Mozzini, Chiara; Guidi, Giancesare; Faccini, G; Cominacini, Luciano

Titolo:

Cigarette smoking blocks the protective expression of Nrf2/ARE pathway in peripheral mononuclear- cells of young heavy smokers favouring inflammation

Anno:

2009

Tipologia prodotto:

Articolo in Rivista

Tipologia ANVUR:

Articolo su rivista

Lingua:

Inglese

Formato:

Elettronico

Referee:

Sì

Nome rivista:

PLoS ONE

ISSN Rivista:

1932-6203

N° Volume:

4

Numero o Fascicolo:

(12) e8225

Intervallo pagine:

1-12

Parole chiave:

cigarette smoking; inflammation; Nrf2; NFkB; oxidative stress; atherosclerosis

Breve descrizione dei contenuti:

Cigarette smoking is an important risk factor for atherosclerosis, a chronic inflammatory disease. However the underlying factors of this effect are unclear. It has been hypothesized that water-soluble components of cigarette smoke can directly promote oxidative stress in vasculature and blood cells. Aim of this study was to study the relationship between oxidative stress and inflammation in a group of young smokers. To do this we evaluated: 1) the oxidation products of phospholipids (oxPAPC) in peripheral blood mononuclear cells (PBMC); 2) their role in causing PBMC reactive oxygen species (ROS) generation and changes in GSH; 3) the expression of the transcription factor NF-E2-related factor 2 (Nrf2) and of related antioxidant genes (ARE); 4) the activation of NF-kB and C-reactive protein (CRP) values. We studied 90 healthy volunteers: 32 non-smokers, 32 moderate smokers (5-10 cigarettes/day) and 26 heavy smokers (25-40 cigarettes/day). OxPAPC and p47phox expression, that reasonably reflects NADPH oxidase activity, were higher in moderate smokers and heavy smokers than in non-smokers (p<0.01), the highest values being in heavy smokers (p<0.01). In in vitro studies oxPAPC increased ROS generation via NADPH oxidase activation. GSH in PBMC and plasma was lower in moderate smokers and heavy smokers than in non-smokers (p<0.01), the lowest values being in heavy smokers (p<0.01). Nrf2 expression in PBMC was higher in moderate smokers than in non-smokers (p<0.01), but not in heavy smokers, who had the highest levels of NF-kB and CRP (p<0.01). In in vitro studies oxPAPC dose-dependently increased NF-kB activation, whereas at the highest concentrations Nrf2 expression was repressed. The small interference (si) RNA-mediated knockdown of NF-kappaB/p65 increased about three times the expression of Nrf2 stimulated with oxPAPC. Cigarette smoke promotes oxPAPC formation and oxidative stress in PBMC. This may cause the activation of NF-kB that in turn may participate in the negative regulation of Nrf2/ARE pathway favouring inflammation.

Pagina Web:

https://doi.org/10.1371/journal.pone.0008225

Id prodotto:

53910

Handle IRIS:

11562/339990

depositato il:

20 marzo 2012

ultima modifica:

8 novembre 2022

Citazione bibliografica:

Garbin, Ulisse; FRATTA PASINI, Anna Maria; Stranieri, Chiara; Cominacini, M; Pasini, Andrea; Manfro, Stefania; Lugoboni, F; Mozzini, Chiara; Guidi, Giancesare; Faccini, G; Cominacini, Luciano, Cigarette smoking blocks the protective expression of Nrf2/ARE pathway in peripheral mononuclear- cells of young heavy smokers favouring inflammation «PLoS ONE» , vol. 4 , n. (12) e8225 , 2009 , pp. 1-12

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